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Updated: Nov 17, 2025
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
A resorufin-based red-emitting fluorescent probe with high selectivity for tracking endogenous peroxynitrite in
Huihui Su1, Nannan Wang1, Jiamin Wang2
1Key Laboratory for Special Functional Materials of Ministry of Education, School of Materials Science and Engineering, Henan University, Kaifeng 475004, PR China.
Abstract:
Peroxynitrite (ONOO-) plays essential roles on various physiological and pathological processes of living systems as a short-lived and highly reactive nitrogen (RNS) specie. The construction of novel long-wavelength fluorescent probes with high specificity towards ONOO- for imaging in vivo is still demand urgently. About this work, a novel resorufin-based red-emitting fluorescent probe for tracking ONOO- has been constructed. The probe RFP exhibited high selectivity towards ONOO- anion over other analytes. Utilizing the probe, ONOO- could be directly observed by the naked eye. Furthermore, RFP was successfully applied for imaging endogenous ONOO- in RAW264.7 cells and inflammatory mice. This work offers a convenient method for monitoring the intercellur ONOO- that be expected to be applied for explaining the bio-functional roles of ONOO- in living system.
