A simple, green chemistry technology for fabrication of tissue-engineered scaffolds based on mussel-inspired 3D...

The fabrication of 3D fibrous scaffolds with highly interconnected pores has been crucial in the development of tissue regeneration techniques. The present study describes the fabrication of 3D fibrous scaffolds by freeze-drying of polydopamine (PDA) coated centrifugal spun gelatin fibers. We wanted to combine the mussel-inspired chemistry, Maillard reaction, and the 3D microstructural advantages of centrifugal spun fibers to develop the green fibrous scaffolds at low cost, high speed, and desired mold shape. The resultant PDA-gelatin fibers exhibited a smooth 3D microstructure with a uniform formation of PDA thin ad-layer that enhanced the mechanical properties and stability of the scaffolds, and thereby decreased the degradation rate. All scaffolds showed promising properties including good dimensional and mechanical stability under wet state, optimal porosity over 94%, and high water uptake of approximately 1500%. The results of cell culture studies, further confirmed that all scaffolds exhibited appropriate biocompatibility, cell proliferation, migration, and infiltration. Particularly, the PDA-coated scaffolds showed a significant enhancement in proliferation, migration, and infiltration of HDF-GFP⁺ cells. These results show that a 3D porous fibrous scaffold with simplifying tunable density and desirable shape on a large scale can be readily prepared for different fields of tissue engineering applications.