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Related Experiment Video

Updated: Nov 17, 2025

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Published on: June 2, 2018

Organoid microphysiological system preserves pancreatic islet function within 3D matrix.

S N Patel¹, M Ishahak², D Chaimov¹

¹J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA.

Science Advances

|February 13, 2021

Summary

This summary is machine-generated.

Microphysiological systems (MPSs) maintain pancreatic organoid function in dynamic culture, outperforming static methods. This technology preserves organoid viability and insulin secretion for extended periods.

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Last Updated: Nov 17, 2025

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

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Published on: June 2, 2018

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Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs

Published on: December 13, 2019

Area of Science:

Biotechnology
Tissue Engineering
In Vitro Models

Background:

Three-dimensional (3D) organoids better model human tissue complexity than 2D cultures.
Standard static culture methods inadequately support organoid physiology.
Microphysiological systems (MPSs) are crucial for maintaining organoid function in vitro.

Purpose of the Study:

To evaluate a polydimethylsiloxane-free MPS for culturing pancreatic organoids.
To assess the impact of dynamic culture on organoid viability and function.
To enable serial, in situ, multiparametric assessments of organoids.

Main Methods:

Cultured human and rodent pancreatic islets within a 3D alginate hydrogel using a novel MPS.
Employed computational modeling to predict hypoxic stress and insulin secretion.
Utilized serial, high-content, noninvasive assessments to monitor organoid health and function.

Main Results:

MPS culture significantly improved organoid viability and function for over 10 days compared to static culture.
Dynamic culture conditions reduced hypoxic stress and enhanced insulin secretion, as predicted.
The MPS platform demonstrated effective serial, in situ, multiparametric assessment capabilities.

Conclusions:

Dynamic in vitro microenvironments are essential for preserving primary organoid function.
The developed MPS is a valuable tool for maintaining organoid physiology and enabling detailed in situ analysis.
This technology advances the utility of organoids for research and drug discovery.