Fluorescein labeling of lymphatic vessels for lymphangiography

A simple technique for identification of pedal lymphatic vessels for lymphangiography was developed. A fluorescein-lidocaine mixture is injected subcutaneously into the foot, and within 20 minutes, local lymphatic vessels absorb the solution and fluoresce vividly under ultraviolet light. These labeled lymphatic channels are readily isolated and cannulated. The technique has proved highly successful, with no documented allergic complications after use in more than 1,000 cases.