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High-resolution quantitative profiling of tRNA abundance and modification status in eukaryotes by mim-tRNAseq

Andrew Behrens1, Geraldine Rodschinka1, Danny D Nedialkova2

  • 1Mechanisms of Protein Biogenesis, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.

Molecular Cell
|February 13, 2021
PubMed

Keywords:
RNA modificationtRNA quantitationtransfer RNAtranslation regulation

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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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