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Klotho, FOXO1 and cytokines associations in patients with coronary artery disease
Toktam Kazemi Fard1, Reza Ahmadi2, Tooran Akbari1
1Department of Biochemistry, Faculty of Basic Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran; Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Insights
This study found reduced Klotho and increased FOXO1 gene expression in atherosclerosis patients. These changes suggest Klotho and FOXO1 may serve as novel biomarkers for atherosclerosis.
Area of Science:
- Biochemistry
- Molecular Biology
- Cardiovascular Research
Background:
- Atherosclerosis is a leading cause of mortality in aging populations.
- Klotho family proteins are implicated in cardiovascular disease, apoptosis, and oxidative stress.
- Understanding gene expression changes in atherosclerosis is crucial for developing new therapies.
Purpose of the Study:
- To investigate alterations in Klotho and FOXO1 gene expression and protein levels in patients with atherosclerosis.
- To explore the potential of Klotho and FOXO1 as biomarkers for atherosclerosis.
Main Methods:
- A case-control study involving 79 atherosclerosis patients and 78 healthy controls.
- Quantitative PCR (qPCR) was used to measure peripheral mono-nuclear blood cells (PBMC) gene expression of Klotho and FOXO1.
- Enzyme-Linked Immunosorbent Assay (ELISA) was employed to determine serum concentrations of Klotho and FOXO1.
Main Results:
- PBMC gene expression of Klotho was significantly reduced in patients compared to controls (P < 0.01).
- PBMC gene expression of FOXO1 was significantly increased in patients compared to controls (P < 0.01).
- Positive correlations were observed between PBMC Klotho gene expression and serum Klotho levels, and between serum Klotho and FOXO1 concentrations.
Conclusions:
- Reduced Klotho and increased FOXO1 expression in PBMCs suggest their involvement in atherosclerosis.
- Klotho and FOXO1 may serve as novel and convenient biomarkers for predicting, prognosing, and monitoring atherosclerosis.
- Further research into these biomarkers could aid in designing targeted drug therapies for atherosclerosis patients.
Introduction:
Atherosclerosis is one of the main reasons for adult mortality in advanced populations and countries with high stress levels. Klotho family are single-pass trans-membrane proteins that involve in the genesis and progression of various diseases, including acardiovascular disease, apoptosis and stress oxidative imbalance. Present study, investigates the pattern of changes in Klotho and FOXO1 gene expressions and levels in atherosclerosis.
Methods:
Present case control study consisted of 79 patients with atherosclerosis and 78 healthy controls. PBMC (peripheral mono-nuclear blood cells) expression levels of Klotho and FOXO1 were assayed, using qPCR method. Serum concentration of Klotho and FOXO1 were measured by ELISA method.
Results:
A significant reduction was found in PBMC genes expression levels of Klotho (P < 0.01) of patients as comparison with controls. PBMC Gene expression of FOXO1 in patients was increased significantly (P < 0.01) when compared with controls. Pearson analysis showed a positive correlation between PBMC Klotho gene expression and Klotho levels of patients (P < 0.01). The correlation between serum concentrations of Klotho and FOXO1 of patients was also positive significantly (P < 0.01). AUC of ROC for gene expression and serum concentration of Klotho in patients were 0.701 and 0.737 respectively.
Conclusion:
Investigating the PBMC gene expression and serum concentration of Klotho in patients with atherosclerosis is suggested could be a convenient novel biomarker for predicting, prognosis, monitoring the disease progression and designing a suitable drug for patients with atherosclerosis.
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