Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Transporters01:31

Membrane Transporters

16.1K
Transporters are essential membrane transport proteins with functions related to cell nutrition, homeostasis, communication, etc. Approximately 7% of all genes in the human genome code for transporters or transporter-related proteins.
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
16.1K
Facilitated Diffusion01:16

Facilitated Diffusion

902
The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
902
The Significance of Membrane Transport01:44

The Significance of Membrane Transport

38.0K
The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
38.0K
Drug Absorption Mechanism: Carrier-Mediated Membrane Transport01:19

Drug Absorption Mechanism: Carrier-Mediated Membrane Transport

5.3K
Certain large, lipid-insoluble drug molecules that resemble amino acids, peptides, or glucose, require specialized carrier proteins to facilitate their diffusion across cell membranes. This transport can occur through either facilitated diffusion, which does not require energy input, or active transport, which does require energy input.
Facilitated diffusion is a passive process that utilizes human Solute Carrier (SLC) transporters. These transporters bind to the drug, undergo structural...
5.3K
Facilitated Transport01:19

Facilitated Transport

142.7K
The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
142.7K
Facilitated Transport01:19

Facilitated Transport

16.6K
The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
16.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resolving three GABA induced electrogenic events and GABA-coupling stoichiometry for Na<sup>+</sup> and Cl<sup>-</sup> in hGAT-1.

The FEBS journal·2026
Same author

Proton-selective conductance and gating of the lysosomal cation channel TMEM175.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Rescue of Epilepsy-Associated Mutations of the Highly Conserved Glycine Residue 443 in the Human GABA Transporter 1.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

Lysosomal Ion Channels and Transporters: Recent Findings, Therapeutic Potential, and Technical Approaches.

Bioelectricity·2025
Same author

Unraveling pH Regulation of TMEM175, an Endolysosomal Cation Channel With a Role in Parkinson's Disease.

Journal of cellular physiology·2025
Same author

Membrane potential stimulates ADP import and ATP export by the mitochondrial ADP/ATP carrier due to its positively charged binding site.

Science advances·2024

Related Experiment Video

Updated: Nov 17, 2025

Introduction to Solid Supported Membrane Based Electrophysiology
19:56

Introduction to Solid Supported Membrane Based Electrophysiology

Published on: May 11, 2013

15.4K

Functional Characterization of SLC Transporters Using Solid Supported Membranes.

Andre Bazzone1, Maria Barthmes2

  • 1Nanion Technologies GmbH, Munich, Germany. andre.bazzone@nanion.de.

Methods in Molecular Biology (Clifton, N.J.)
|February 14, 2021
PubMed
Summary

We developed a cost-effective, high-throughput electrophysiology protocol for characterizing the human peptide transporter PepT1 and other membrane transporters. This method enables efficient functional analysis of transporter activity and inhibition.

Keywords:
EAAT3Membrane transporterNCXOCT2PepT1SGLT1SLC transporterSSMSURFE2RSURFERSSM-based electrophysiologyTransporter assay

More Related Videos

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology
09:12

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology

Published on: May 3, 2021

2.8K
A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
07:47

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

Published on: April 20, 2015

10.1K

Related Experiment Videos

Last Updated: Nov 17, 2025

Introduction to Solid Supported Membrane Based Electrophysiology
19:56

Introduction to Solid Supported Membrane Based Electrophysiology

Published on: May 11, 2013

15.4K
Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology
09:12

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology

Published on: May 3, 2021

2.8K
A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
07:47

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

Published on: April 20, 2015

10.1K

Area of Science:

  • Membrane biophysics
  • Molecular pharmacology
  • Electrophysiology

Background:

  • Functional characterization of membrane transporters is crucial for understanding cellular transport mechanisms.
  • Existing methods can be costly and time-consuming, limiting high-throughput analysis.
  • Solid supported membrane (SSM)-based electrophysiology offers a promising alternative.

Purpose of the Study:

  • To present a protocol for the functional characterization of the H+-coupled human peptide transporter PepT1.
  • To provide guidelines for adapting the protocol to other transporters like SGLT1, OCT2, NCX, and EAAT3.
  • To demonstrate the utility of SSM-based electrophysiology for transporter research.

Main Methods:

  • Development of an SSM-based electrophysiology assay using the SURFE2R N1 instrument.
  • Utilizing SSM-coated gold sensors for adsorbing membrane vesicles.
  • Applying substrate concentration jumps to activate transporter activity and measuring capacitive currents.

Main Results:

  • The protocol successfully characterized PepT1-mediated glycylglycine transport.
  • EC50 for PepT1-mediated transport was determined.
  • An inhibition experiment using a specific peptide inhibitor demonstrated the assay's sensitivity.

Conclusions:

  • SSM-based electrophysiology provides a cost-effective, user-friendly, and high-throughput method for functional transporter characterization.
  • The presented protocol is adaptable for a range of transporters, facilitating broader research applications.
  • This technique enables detailed analysis of transporter kinetics and inhibition profiles.