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Crosslinked insulins: preparation, properties, and application.

D Brandenburg, H G Gattner, W Schermutzki

Advances in Experimental Medicine and Biology

|January 1, 1977

Summary

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Crosslinked insulin derivatives are useful for studying insulin structure and function. New crosslinked insulin monomers and dimers were synthesized and characterized, aiding in structure-function investigations.

Area of Science:

Biochemistry
Molecular Biology
Endocrinology

Background:

Crosslinked insulins are valuable tools for structure-function studies and as proinsulin models.
Previous research has focused on analytical investigations, preparation, biological activities, and spectral properties of crosslinked insulin derivatives.

Purpose of the Study:

To review existing literature on crosslinked insulins.
To synthesize and characterize new A1-B29-crosslinked insulin monomers and symmetrical dimers.
To investigate the properties of these new insulin derivatives through tritium-labeling and enzymatic degradation.

Main Methods:

Literature review of analytical investigations, preparation, biological activities, and CD-spectral properties.
Synthesis of new A1-B29-crosslinked insulin monomers and symmetrical dimers (A1-A'1, B1-B'1, B29-B'29 linked).

Related Experiment Videos

Tritium-labeling and enzymatic degradation experiments on A1-B29-linked insulins.

Main Results:

Summarized results of reduction/reoxidation studies on insulin derivatives with irreversible and cleavable crosslinks.
Described new A1-B29-crosslinked monomers and three symmetrical dimers.
Presented initial findings from tritium-labeling and enzymatic degradation experiments.

Conclusions:

Crosslinked insulins, including newly synthesized monomers and dimers, offer versatile platforms for structure-function relationship studies.
These derivatives provide insights into insulin's behavior under various conditions, including enzymatic degradation.