Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Clonal evolution in human lymphoblast cultures.

B R Migeon1, J Axelman, G Stetten

  • 1Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD.

American Journal of Human Genetics
|May 1, 1988
PubMed
Summary

Lymphoblast cultures from heterozygous females lose their X-linked phenotype over time. This evolution toward single-cell populations means cultures may not reflect the original cell

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Compound dependence of the relationship log K(ow) and log BCF L : A comparison between chlorobenzenes (CBs) for rainbow trout and polycyclic aromatic hydrocarbons (PAHs) forDaphnia.

Environmental science and pollution research international·2013
Same author

Redefining the risks of prenatally ascertained supernumerary marker chromosomes: a collaborative study.

Journal of medical genetics·2006
Same author

Observations of the PCB distribution within and in-between ice, snow, ice-rafted debris, ice-interstitial water, and seawater in the Barents Sea marginal ice zone and the North Pole area.

The Science of the total environment·2005
Same author

X chromosome inactivation: theme and variations.

Cytogenetic and genome research·2003
Same author

Real-time three-dimensional ultrasound methods for shape analysis and visualization.

Methods (San Diego, Calif.)·2002
Same author

Second trimester prenatal diagnosis of epignathus teratoma in ring X chromosome mosaicism with inactive ring X chromosome.

Annales de genetique·2002

Area of Science:

  • Genetics
  • Cell Biology
  • X-chromosome inactivation

Background:

  • Normal females heterozygous for X-linked genetic markers exhibit mosaicism due to X-chromosome inactivation.
  • Lymphoblast cultures are utilized to study cellular evolution and genetic expression.

Purpose of the Study:

  • To investigate the evolutionary dynamics of lymphoblast cultures derived from heterozygous females.
  • To determine if lymphoblast cultures maintain their heterozygous X-linked phenotype over time.

Main Methods:

  • Established lymphoblast cultures from normal females heterozygous for glucose-6-phosphate dehydrogenase (G6PD) variants.
  • Monitored the expression of X-linked markers, specifically G6PD isozymes, through serial subculturing.

Main Results:

  • Initially, cultures displayed a heterozygous phenotype reflecting mosaic cell populations.
  • By the tenth subculture, 50% of the cultures lost their heterozygous phenotype, expressing only a single G6PD isozyme.
  • Cellular composition evolution was influenced by initial cell composition, allele-specific growth advantages/disadvantages, and stochastic events.

Conclusions:

  • Lymphoblast cultures can undergo significant evolution, potentially losing their original heterozygous X-linked phenotype.
  • The observed shift suggests a tendency toward clonal expansion within lymphoblast cultures.
  • These findings highlight the importance of considering cellular evolution when interpreting results from lymphoblast cultures.

Related Experiment Videos