Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genetic Features of the Scuticociliate Pathogen Philaster sp. Isolate FWC2 That Causes Sea Urchin Mass Mortality.

The Journal of eukaryotic microbiology·2026

Same author

Composition of fish egg assemblages varies with depth on the West Florida Shelf.

PeerJ·2026

Same author

Draft genome sequence of <i>Gracilimonas</i> sp. strain BCB1 isolated from the gill tissue of the lucinid bivalve <i>Stewartia floridana</i> in Pinellas County, Florida, USA.

Microbiology resource announcements·2025

Same author

Prophage regulation of <i>Shewanella fidelis</i> 3313 motility and biofilm formation with implications for gut colonization dynamics in <i>Ciona robusta</i>.

eLife·2025

Same author

A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid colorimetric detection of pepper mild mottle virus (PMMoV).

Journal of virological methods·2025

Same author

Diverse ssRNA viruses associated with <i>Karenia brevis</i> harmful algal blooms in southwest Florida.

mSphere·2025

Search research articles

Related Experiment Video

Updated: Nov 17, 2025

DNA-based Fish Species Identification Protocol

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

DNA Detectives: Outreach Activity Teaching Students to Identify Fish Eggs Using DNA Barcoding

Makenzie Kerr¹, Mya Breitbart¹

¹University of South Florida, Saint Petersburg, FL 33701.

Journal of Microbiology & Biology Education

|February 15, 2021

Summary

No abstract available in PubMed .

More Related Videos

Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria

Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria

Published on: July 20, 2017

Related Experiment Videos

Last Updated: Nov 17, 2025

DNA-based Fish Species Identification Protocol

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria

Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria

Published on: July 20, 2017