Vascularization and osteogenesis in ectopically implanted bone tissue-engineered constructs with endothelial and
Jelena G Najdanović1, Vladimir J Cvetković2, Sanja T Stojanović1
1Department of Biology and Human Genetics; Department for Cell and Tissue Engineering, Faculty of Medicine, University of Niš, Niš 18108, Serbia.
World Journal of Stem Cells
|February 15, 2021
Summary
Bone tissue engineering constructs combining endothelial and osteogenic cells with platelet-rich plasma show promise for bone defect healing. Further research is needed to optimize cell ratios and prevent tissue regression for clinical use.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Cell Biology
Background:
- Bone defect healing is hindered by poor vascularization.
- Endothelial cells (ECs) and osteoblasts (OBs) interplay is crucial for bone regeneration.
- Platelet-rich plasma (PRP) serves as a reservoir for growth factors.
Purpose of the Study:
- To evaluate vascularization and osteogenesis in bone tissue engineering constructs.
- To investigate constructs enriched with PRP and adipose-derived stem cells (ASCs) differentiated into ECs and OBs.
Main Methods:
- Adipose-derived stem cells (ASCs) were differentiated into ECs and OBs in vitro.
- Constructs included differentiated ASCs with PRP and bone matrix (BPEO), uninduced ASCs with PRP (BPUI), or bone matrix alone (BC).
- Gene expression and histological analyses were performed at various time points post-implantation in mice.
Main Results:
- BPEO constructs showed favorable vascularization and osteogenesis, with high CD31 expression.
- BPUI constructs exhibited increased endothelial gene expression and osteocalcin levels compared to controls.
- Tissue regression was observed in BPEO constructs after 8 weeks, indicating a need for optimization.
Conclusions:
- Ectopic implantation of BPEO constructs positively influenced vascularization and osteogenesis.
- Tissue regression necessitates further investigation into optimal EC/OB ratios for clinical translation.
- The study highlights the potential of combined cell therapies in bone tissue engineering.
Keywords:
Adipose-derived stem cellsBone-related genesEctopic osteogenesisEndothelial-related genesPlatelet-rich plasmaVascularization
