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Comparative leukocyte esterase-nitrite and BAC-T-SCREEN studies using single and multiple urine volumes
J J Damato1, J Garis, R J Hawley
1Department of Pathology, Walter Reed Army Medical Center, Washington, DC 20307-5100.
Archives of Pathology & Laboratory Medicine
|May 1, 1988
Summary
The BAC-T-SCREEN filtration system is superior for detecting microorganisms in urine compared to Chemstrip LN. Analyzing multiple urine volumes significantly enhances BAC-T-SCREEN sensitivity and accuracy for microbial detection.
Area of Science:
- Clinical Microbiology
- Diagnostic Testing
Background:
- Rapid detection of microorganisms in urine is crucial for timely diagnosis and treatment of urinary tract infections.
- Existing diagnostic methods may have limitations in sensitivity and specificity.
Purpose of the Study:
- To compare the performance of the Chemstrip LN and BAC-T-SCREEN filtration systems for rapid urine microorganism detection.
- To evaluate the impact of analyzing multiple urine volumes on BAC-T-SCREEN test performance.
Main Methods:
- 980 urine specimens were analyzed using both Chemstrip LN and BAC-T-SCREEN systems.
- BAC-T-SCREEN was tested with multiple urine volumes (1 mL, 2 mL, 3 mL) to assess performance enhancement.
- Specimen processability was assessed, noting exclusions due to interfering substances or clogging.
Main Results:
- Chemstrip LN showed a sensitivity of 48% and specificity of 93%.
- BAC-T-SCREEN performance improved with increased urine volume: sensitivity ranged from 88% to 98%, specificity from 95% to 98%.
- Analyzing multiple urine volumes allowed detection of 92% of samples with low colony-forming units (≤10^4).
Conclusions:
- The BAC-T-SCREEN system demonstrates superior performance over Chemstrip LN for detecting urine microorganisms.
- Utilizing multiple urine volumes with the BAC-T-SCREEN system significantly enhances test sensitivity and diagnostic accuracy.