Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

T- and B-cells in immunologic diseases.

S Strober

    American Journal of Clinical Pathology
    |November 1, 1977
    PubMed
    Summary

    This study details methods for accurately quantifying human peripheral blood lymphocytes, specifically thymus-derived (T) and bursa-equivalent (B) cells. Accurate cell counts are crucial for diagnosing various immune disorders and conditions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A Cost Analysis of Tolerance Induction for Two-Haplotype Match Kidney Transplant Recipients.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
    Same author

    Allogeneic hematopoietic cell transplantation after failed autologous transplant for lymphoma using TLI and anti-thymocyte globulin conditioning.

    Bone marrow transplantation·2015
    Same author

    Chimerism, graft survival, and withdrawal of immunosuppressive drugs in HLA matched and mismatched patients after living donor kidney and hematopoietic cell transplantation.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
    Same author

    Requirement for interactions of natural killer T cells and myeloid-derived suppressor cells for transplantation tolerance.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2014
    Same author

    Tolerance and withdrawal of immunosuppressive drugs in patients given kidney and hematopoietic cell transplants.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2012
    Same author

    Surface plasmon immunoassay.

    Applied optics·2010

    Area of Science:

    • Immunology
    • Hematology
    • Cell Biology

    Background:

    • Human peripheral blood contains three main lymphocyte subpopulations: T lymphocytes, B lymphocytes, and null cells.
    • T-lymphocytes are identified by sheep erythrocyte receptors and specific antisera, while B-lymphocytes are identified by surface immunoglobulin or C3 receptors.
    • Null cells function as killer (K) cells in antibody-dependent cellular cytotoxicity and possess Fc receptors.

    Purpose of the Study:

    • To address challenges in accurately quantifying T- and B-lymphocytes in peripheral blood.
    • To highlight the clinical significance of precise lymphocyte quantitation in disease diagnosis.

    Main Methods:

    • Identification of T-lymphocytes using sheep erythrocyte rosettes and specific heterologous antisera.
    • Enumeration of B-lymphocytes via immunofluorescence for surface immunoglobulin or C3 receptor assays.
    • Characterization of null cells by Fc receptor presence and K-cell activity.

    Main Results:

    • Accurate quantitation of T- and B-cells is often hindered by monocyte contamination, immunoglobulin binding, and disease-related surface marker changes.
    • Despite these challenges, reliable assays for T- and B-cells are essential for clinical diagnostics.

    Conclusions:

    • Accurate quantification of peripheral blood T- and B-lymphocytes is vital for diagnosing conditions like lymphocytosis, immunodeficiency disorders, transplant rejection, and Hodgkin's disease.
    • Improved methods for lymphocyte enumeration hold significant clinical value.

    Related Experiment Videos