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Related Experiment Video

Updated: Nov 17, 2025

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Programmable tools for targeted analysis of epigenetic DNA modifications.

Benjamin Buchmuller¹, Anne Jung¹, Álvaro Muñoz-López¹

¹Faculty of Chemistry and Chemical Biology, TU Dortmund University Otto-Hahn-Str. 4a, 44227 Dortmund, Germany.

Current Opinion in Chemical Biology

|February 15, 2021

Summary

New probes enable targeted analysis of dynamic epigenetic marks on DNA. These tools allow high-resolution study of modified nucleobases in vitro and in situ, aiding research in development and disease.

Keywords:

Active DNA demethylation DNA methylation Epigenetics Molecular recognition Programmable DNA-binding probes TET dioxygenases

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Published on: December 18, 2017

Area of Science:

Epigenetics and Molecular Biology
Genomics and DNA Modifications

Background:

Cytosine 5-position modifications are dynamic epigenetic marks in mammalian DNA.
These epigenetic marks play crucial roles in biological development and disease.
Analyzing these modified nucleobases is key to understanding their functions.

Purpose of the Study:

To review emerging methods for targeted analysis of epigenetically modified nucleobases.
To highlight the development of novel probes for analyzing DNA modifications.
To explore the potential of these probes for high-resolution epigenetic studies.

Main Methods:

Review of recent advancements in probe development for nucleobase analysis.
Description of probes combining DNA-binding domains with modified nucleobase recognition.
Focus on targeted, sequence-specific epigenetic analyses.

Main Results:

Probes enable simple, high-resolution analysis of modified nucleobases in vitro.
Probes facilitate in situ correlation of nucleobases with chromatin regulatory elements.
Single-cell imaging allows user-defined locus analysis of epigenetic marks.

Conclusions:

Emerging probes offer powerful tools for targeted epigenetic analysis.
These methods advance the understanding of DNA modifications in biological processes.
High-resolution, single-cell analysis capabilities open new research avenues.