Effect of pH on Isoliquiritigenin (ISL) fluorescence in lipo- polymeric system and metallic nanosystem

Flavonoids have various medicinal properties such as anti-inflammatory, anti-oxidant, anti-cancer, antiviral. Yet, the fluorescent properties of flavonoids are less explored and termed as autofluorescence in general. This study investigates the fluorescence properties of Isoliquiritigenin (ISL) in various alkaline conditions. The maximum fluorescence emission was obtained at pH 12 on excitation wavelength of 440 nm. Theoretical and experimental investigation on the shift in UV-Vis absorbance spectra, upon the variation in pH, performed, indicated deprotonation as the cause. PEG-based stable liposome carrier, with an internal alkaline environment (LIP-ISL-NaOH) that aids in flavonoid fluorescence, was synthesized using a modified thin-film hydration method. The hydrodynamic size of the liposome synthesized was in the range of 50-70 nm. PEG, on the addition, found maintaining the alkaline environment in the internal chamber of the lipo-polymer system, helps the LIP-ISLNaOH nanosystem to exhibit fluorescence irrespective of the suspension pH. Further, reducing property of ISL was used for the synthesis of Au nanoclusters to achieve theranostic nature.