Related Experiment Video
Updated: Nov 17, 2025
Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
A workflow for simultaneous DNA copy number and methylome analysis of inner cell mass and trophectoderm cells from
Meir Olcha1, Xiao Dong2, Heather Feil1
1Department of Obstetrics and Gynecology, Montefiore Medical Center/Albert Einstein College of Medicine, Bronx, New York.
Objective:
To establish a workflow for isolating single trophectoderm (TE) and inner cell mass (ICM) cells and to simultaneously evaluate these cells for copy number variation (CNV) as well as methylome development.
Design:
Experimental.
Setting:
Academic medical center.
Patient(S):
Donated genetically abnormal blastocysts.
Intervention(S):
Single cells were isolated, followed by bisulfite conversion and sequencing to identify CNV and methylome profiles.
Main Outcome Measure(S):
CNV and methylation profiling.
Result(S):
Two embryos were dissociated, isolating 46 single cells, with 17 ICM and 12 TE cells selected for further downstream analysis. Chromosome ploidies and embryo sex were concordant with the results from conventional aneuploidy testing. In 3 of the 29 cells, additional aneuploidies were discovered, indicating possible mosaicism undetected by routine preimplantation genetic testing for aneuploidy. CpG methylation frequency was higher in ICM cells compared with TE cells (44.3% vs. 32.4%), respectively, while non-CpG methylation frequency was similar among both cell types. CpG methylation levels accurately distinguished ICM from TE cells epigenetically.
Conclusion(S):
We describe an effective workflow for isolating and sequencing single ICM and TE cells from human blastocysts. The use of methylation profiling can help distinguish these two cell populations better then morphologic identification alone. TE cells had significantly lower levels of DNA methylation, which may be explained in part by the fact that these cells have begun the process of differentiation and are transcriptionally more active than ICM. This approach may be used to explore the genetic complexities within human embryos, specifically among the two primary cell types seen at this stage of development.
