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Proteome-wide and matrisome-specific alterations during human pancreas development and maturation
Zihui Li1, Daniel M Tremmel2, Fengfei Ma3
1Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
Nature Communications
|February 16, 2021
Summary
This study reveals new insights into the extracellular matrix (ECM) of the human pancreas across development. We identified key ECM proteins and their changes, improving our understanding of pancreatic maturation.
Area of Science:
- Biochemistry
- Developmental Biology
- Proteomics
Background:
- The extracellular matrix (ECM) is crucial for tissue development and function, but its proteome during human pancreas development is poorly understood.
- Systematic studies of ECM changes throughout pancreatic maturation are lacking.
Purpose of the Study:
- To comprehensively analyze the proteome-wide and ECM-specific alterations in the human pancreas across different developmental stages.
- To identify novel proteome and matrisome features during pancreas development and maturation.
Main Methods:
- Utilized mass spectrometry-based quantitative proteomics with N,N-dimethyl leucine isobaric tags.
- Analyzed samples from four distinct age groups: fetal, juvenile, young adult, and older adult.
- Employed immunofluorescent staining to visualize ECM protein localization.
Main Results:
- Identified 3,523 proteins, including 185 ECM proteins, with 117 quantified.
- Detected previously unrecognized proteome and matrisome characteristics during pancreatic development.
- Observed changes in ECM protein localization within islet and acinar compartments.
Conclusions:
- This study provides a detailed proteomic map of the human pancreatic ECM throughout development.
- The findings enhance our understanding of the ECM's dynamic role in pancreas maturation and function.
- Reveals novel ECM components and localization patterns critical for pancreatic development.
