Related Experiment Video
Updated: Nov 17, 2025

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
Published on: June 17, 2012
Identification of the kinanthraquinone biosynthetic gene cluster by expression of an atypical response regulator
Risa Takao1,2, Katsuyuki Sakai2, Hiroyuki Koshino3
1Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama-shi, Saitama, Japan.
Abstract:
Recent advances in genome sequencing have revealed a variety of secondary metabolite biosynthetic gene clusters in actinomycetes. Understanding the biosynthetic mechanism controlling secondary metabolite production is important for utilizing these gene clusters. In this study, we focused on the kinanthraquinone biosynthetic gene cluster, which has not been identified yet in Streptomyces sp. SN-593. Based on chemical structure, 5 type II polyketide synthase gene clusters were listed from the genome sequence of Streptomyces sp. SN-593. Among them, a candidate gene cluster was selected by comparing the gene organization with grincamycin, which is synthesized through an intermediate similar to kinanthraquinone. We initially utilized a BAC library for subcloning the kiq gene cluster, performed heterologous expression in Streptomyces lividans TK23, and identified the production of kinanthraquinone and kinanthraquinone B. We also found that heterologous expression of kiqA, which belongs to the DNA-binding response regulator OmpR family, dramatically enhanced the production of kinanthraquinones.
More Related Videos
10:10Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
Published on: May 29, 2010
09:08From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Related Concept Videos
Cell Signaling in Plants
Gene Regulation in Microbial Communities: Quorum Sensing
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Global Regulatory Systems
Gene Regulation During Sporulation
Repressible Operon: trp Operon