[Mechanism of action of anesthetics--ketamine derivatives--on phospholipid membranes]

Fluorescent probes were applied in the studies of ketamine derivatives interaction with model phospholipid membranes (liposomes). It has been found that kalipsol and ketalar have no effects on the lipid phase viscosity, but increase markedly a positive surface membrane charge. The action of these substances manifests some seconds after the addition to the membrane suspension. The binding constant of ketamine (Cb = 1.2 X 10(3) M-1) and the number of binding sites (N = 1 mol per 7.5 mol of phosphatidyl-choline) have been calculated.