Preparation and characterization of chitosan/Nano-ZnO composite film with antimicrobial activity

Chitosan is promising material for making food packaging film with antimicrobial activity. However, chitosan film usually has limited mechanical and antimicrobial properties and higher water solubility. To improve the performance of chitosan film, in this work, chitosan composite films were prepared by incorporating different sizes of zinc oxide particles of 5 μm, 100 nm, and 50 nm. The films were characterized by scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, and mechanical analysis. Antimicrobial assay of the chitosan and CTS/nano-ZnO composite films against Escherichia coli and Staphylococcus aureus show that the composite chitosan films have better antibacterial activity. The film containing 0.3% of 50 nm zinc oxide particles showed the best inhibition rate, suggesting that smaller sizes of nano-ZnO particles have better bacteriostatic activity and potent application as an antibacterial additive ingredient.