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Glucocorticoid binding sites in human temporal cortex.

A Sarrieau1, M Dussaillant, R M Sapolsky

  • 1Douglas Hospital Research Center, Department of Psychiatry, McGill University, Montreal, Canada.

Brain Research
|February 23, 1988
PubMed
Summary
This summary is machine-generated.

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Researchers found glucocorticoid receptors in the human temporal cortex, suggesting a way these hormones regulate brain function. This discovery is key for understanding neuroendocrine effects in the brain.

Area of Science:

  • Neuroscience
  • Endocrinology
  • Molecular Biology

Background:

  • Glucocorticoids are critical hormones influencing various physiological processes, including brain function.
  • Understanding how glucocorticoids interact with the human brain is essential for neurobiology and medicine.

Purpose of the Study:

  • To investigate the presence and characteristics of glucocorticoid binding sites in the human temporal cortex.
  • To explore the potential intracellular mechanisms of glucocorticoid action in human cortical neurons.

Main Methods:

  • Human temporal cortex tissue samples were obtained from epileptic patients undergoing partial lobectomy.
  • Radioligand binding assays were performed using [3H]dexamethasone to quantify glucocorticoid binding sites.
  • Competitive binding experiments were conducted using various unlabeled steroids to determine receptor affinity and specificity.

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Main Results:

  • Glucocorticoid binding sites were identified in the human temporal cortex.
  • The binding exhibited an apparent dissociation constant (Kd) of approximately 2.8 nM and a maximum binding capacity (Bmax) of approximately 34 fmol/mg protein.
  • Steroid competition assays revealed a specific binding profile, with RU 28362, RU 38486, cortisol, and dexamethasone showing high affinity.

Conclusions:

  • The human temporal cortex possesses specific intracellular glucocorticoid binding sites.
  • These findings support an intracellular mechanism for glucocorticoid regulation of neuronal function in the human cortex.
  • This research provides a molecular basis for the neuroendocrine effects of glucocorticoids in the human brain.