Direct MALDI-TOF profiling of gingival crevicular fluid sediments for periodontitis diagnosis

Periodontitis is a widespread stomatological disease and represents one of the main causes of tooth loss in adults. Traditional diagnosis of periodontitis relies on the judgment by professional periodontists that cannot reveal its progression at the early stage. In this work, we characterized the gingival crevicular fluid (GCF) sediments of patients with periodontitis and healthy volunteers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Potential protein biomarkers were selected based on the multivariate statistical analysis of the MALDI-TOF mass spectra, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification. Twelve potential protein biomarkers were identified from 17 patients compared to 7 healthy volunteers, including 5 microbial proteins and 7 human proteins, indicating the microbial composition and host response components related to the etiology of periodontitis. The panel of biomarkers was then verified with the GCF samples of another 11 patients. The 12 biomarkers also showed potential value in the early diagnosis of periodontitis. This work developed a rapid assay to screen periodontitis among populations. It can be popularized to non-periodontal specialists such as community general practitioners, benefiting the early and accurate monitoring of periodontitis. The identification of the potential biomarkers can also help in the understanding of the pathogenesis of periodontitis.