Related Experiment Video
Updated: Nov 17, 2025
Colon Ascendens Stent Peritonitis CASP - a Standardized Model for Polymicrobial Abdominal Sepsis
Published on: December 18, 2010
Matrix metalloproteinase-9 -1562 C/T polymorphism is associated with the risk of sepsis in a Chinese population: A
Cuijuan Zheng1, Jiayu Wang1, Shouxiang Xie2
1Department of Anaesthesiology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, China.
Abstract:
Matrix metalloproteinase-9 (MMP-9) has been shown to participate in the pathogenesis of sepsis. In this study, we recruited 312 sepsis patients and 413 controls to explore the relationship between sepsis risk and the MMP-9 -1562 C/T polymorphism in Han Chinese. The PCR restriction fragment length polymorphism method was used for genotyping. Our data indicated that the MMP-9 -1562 C/T polymorphism was related with the risk of sepsis (CT vs. CC: P = 0.033, odds ratio (OR) = 1.45, 95% confidence interval (CI) 1.03-2.05; TT+CT vs. CC: P = 0.019, OR = 1.49, 95% CI 1.07-2.07). Stratified analyses demonstrated that this effect was more evident in smokers, drinkers, females and overweight individuals. Furthermore, cross-over analyses suggested that the combined effect of smoking and CT genotype of -1562 C/T polymorphism contributed to the risk of sepsis. In addition, MMP-9 serum levels were significantly lower in sepsis patients than in controls. The MMP-9 -1562 C/T polymorphism was significantly associated with decreased MMP-9 serum levels. Lastly, we observed that this polymorphism was connected to the mortality of sepsis. In conclusion, the interaction between the MMP-9 -1562 C/T polymorphism and smoking correlated with the risk of sepsis in Han Chinese. This polymorphism may serve as a diagnostic marker for sepsis patients.
Insights
The MMP-9 -1562 C/T gene variant increases sepsis risk, particularly in smokers. This polymorphism is linked to lower MMP-9 levels and sepsis mortality, suggesting its potential as a diagnostic marker.
Area of Science:
- Genetics
- Molecular Biology
- Critical Care Medicine
Background:
- Matrix metalloproteinase-9 (MMP-9) plays a role in sepsis pathogenesis.
- Genetic variations in MMP-9 may influence sepsis susceptibility and outcomes.
Purpose of the Study:
- To investigate the association between the MMP-9 -1562 C/T polymorphism and sepsis risk in the Han Chinese population.
- To explore the relationship between this polymorphism, MMP-9 serum levels, and sepsis mortality.
Main Methods:
- Genotyping of 312 sepsis patients and 413 controls using PCR restriction fragment length polymorphism.
- Analysis of MMP-9 serum levels and correlation with genotype.
- Assessment of sepsis risk and mortality in relation to the MMP-9 polymorphism.
Main Results:
- The MMP-9 -1562 C/T polymorphism was significantly associated with increased sepsis risk (OR=1.45-1.49).
- This association was more pronounced in smokers, drinkers, females, and overweight individuals.
- The polymorphism correlated with lower MMP-9 serum levels and increased sepsis mortality. Smoking combined with the CT genotype heightened sepsis risk.
Conclusions:
- The MMP-9 -1562 C/T polymorphism is a risk factor for sepsis in Han Chinese, especially when interacting with smoking.
- This genetic variant may serve as a potential biomarker for sepsis diagnosis and prognosis.
