Ozonation Treatment Increases Chlorophenylacetonitrile Formation in Downstream Chlorination or Chloramination
Di Zhang1,2, Tom Bond3, Mingli Li1,2
1State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze River Water Environment, Ministry of Education, International Joint Research Center for Sustainable Urban Water System, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Chlorophenylacetonitriles (CPANs), emerging drinking water disinfection byproducts, are poorly removed and even increased by ozonation. This study identifies low-molecular-weight protein fragments, specifically tyrosine and tryptophan amino acids, as key precursors for CPAN formation.
Area of Science:
- Environmental Chemistry
- Water Treatment Science
- Organic Chemistry
Background:
- Chlorophenylacetonitriles (CPANs) are emerging aromatic nitrogenous disinfection byproducts (DBPs) in drinking water.
- Limited understanding of CPAN precursors and formation pathways impedes effective control strategies.
Purpose of the Study:
- To screen for CPAN precursors in real drinking water samples.
- To investigate the role of protein fragments, specifically amino acids, in CPAN formation.
- To elucidate CPAN formation pathways during chlor(am)ination.
Main Methods:
- Formation potential (FP) tests on drinking water samples from six treatment plants.
- Ozonation and fluorescence spectroscopy to assess precursor changes.
- High-resolution mass spectrometry and frontier molecular orbital theory for pathway elucidation.
- Chlorination/chloramination experiments with model amino acid precursors (tyrosine, tryptophan).
Main Results:
- Average CPAN precursor removal across treatment plants was only 10%.
- Ozonation significantly increased CPAN precursors by 140% while reducing aromatic proteins.
- Low-apparent-molecular-weight (<1 kDa) substances correlated with CPAN FP.
- Tyrosine and tryptophan were identified as novel CPAN precursors.
Conclusions:
- Low-molecular-weight protein fragments, particularly aromatic amino acids like tyrosine and tryptophan, are significant precursors for CPANs.
- Ozonation may enhance CPAN formation potential by generating these precursors.
- Understanding these pathways provides a theoretical basis for controlling CPANs in drinking water.
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