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Immunophenotypes by flow cytometry: a longitudinal study in healthy individuals.

P C Bishop1, D Boone, J W Parker

  • 1Department of Pathology, University of Southern California School of Medicine, Los Angeles 90033.

Diagnostic and Clinical Immunology
|January 1, 1988
PubMed
Summary

This study tracked immune cell variations in healthy volunteers over 13 weeks. Minor week-to-week changes were observed in most immune cell phenotypes, suggesting stability with some environmental influence.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Understanding immune cell phenotype stability is crucial for interpreting immunological studies.
  • Peripheral blood lymphocytes and monocytes are key components of the adaptive and innate immune systems.

Purpose of the Study:

  • To assess the week-to-week variability of immunological phenotypes in peripheral blood lymphocytes and monocytes.
  • To identify specific immune cell populations that exhibit significant fluctuations over a 13-week period.

Main Methods:

  • Collected weekly blood samples from five healthy volunteers over 13 weeks.
  • Utilized two-color flow cytometry to analyze eleven lymphocyte and two monocyte antigens.
  • Quantified variations using monoclonal antibodies against specific cell surface markers.

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Main Results:

  • Average week-to-week differences were below 5% for most evaluated monoclonal antibodies and cell combinations.
  • Significant variations were noted for I2+, NKH-1+, MO1+, and MO2+ cell populations.
  • These fluctuations suggest a combination of technical variability and immune system responses to environmental factors.

Conclusions:

  • Peripheral blood lymphocyte and monocyte phenotypes exhibit a high degree of stability over a 13-week period in healthy individuals.
  • Specific immune cell subsets (I2+, NKH-1+, MO1+, MO2+) show greater variability, warranting further investigation.
  • Observed variations likely result from a interplay between experimental consistency and the immune system's adaptation to daily environmental exposures.