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Urinary Exosomal microRNA-21 as a Marker for Scrub Typhus-Associated Acute Kidney Injury

Chi Young Yun1, Ji Hye Lim2, Ju Hwan Oh2

  • 1Christian Medical Research Center, Presbyterian Medical Center, Jeonju, Korea.

Genetic Testing and Molecular Biomarkers
|February 17, 2021
PubMed

Insights

Urinary exosomal microRNA-21 (miRNA-21) shows promise as a diagnostic biomarker for acute kidney injury (AKI) in scrub typhus patients. Elevated levels of urinary exosomal miRNA-21 can help detect kidney disease associated with scrub typhus.

Area of Science:

  • Nephrology
  • Biomarker Discovery
  • Infectious Diseases

Background:

  • Acute kidney injury (AKI) is a significant complication in scrub typhus patients.
  • Current diagnostic methods for AKI may have limitations.
  • Urinary microRNA-21 (miRNA-21) is recognized as a potential biomarker for AKI.

Purpose of the Study:

  • To investigate the clinical significance of urinary exosomal miRNA-21 levels in scrub typhus patients.
  • To determine if urinary exosomal miRNA-21 can serve as a novel diagnostic approach for detecting kidney disease.
  • To evaluate the diagnostic performance of urinary exosomal miRNA-21 for scrub typhus-associated AKI.

Main Methods:

  • Collected 138 urine samples from scrub typhus patients at admission.
  • Assessed urinary exosomal miRNA-21 levels in 25 matched patients with and without AKI.
  • Analyzed correlations between miRNA-21 levels, leukocyte counts, and estimated glomerular filtration rate (eGFR).
  • Utilized receiver operating characteristic (ROC) curve analysis for diagnostic assessment.

Main Results:

  • AKI patients exhibited higher total leukocyte counts compared to non-AKI patients.
  • Urinary exosomal miRNA-21 levels were significantly higher in the AKI group.
  • miRNA-21 levels showed a direct correlation with leukocyte counts and an inverse correlation with eGFR.
  • ROC curve analysis indicated good discriminative power for scrub typhus-associated AKI (AUC = 0.907).

Conclusions:

  • Urinary exosomal miRNA-21 is a potential surrogate marker for the diagnosis of scrub typhus-associated AKI.
  • This finding suggests a novel non-invasive diagnostic approach for kidney injury in scrub typhus.
  • Further research is warranted to validate these findings in larger cohorts.
Keywords:
acute kidney injuryexosomemicroRNAscrub typhusurine

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