Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chartreusin: production and microbiological assay.

L J Hanka, S A Gerpheide

    Antimicrobial Agents and Chemotherapy
    |November 1, 1977
    PubMed
    Summary

    Adding d-fucose to fermentation media significantly boosts chartreusin production by Streptomyces chartreusis. This optimization enhances antibiotic yields for potential therapeutic applications.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Determination of diethylstilbestrol in feed supplements by microbiological assay.

    Antibiotics & chemotherapy (Northfield, Ill.)·2014
    Same author

    Psicofuranine. IV. Microbiological assay.

    Antibiotics & chemotherapy (Northfield, Ill.)·2014
    Same author

    Methods for isolation of streptoverticillia from soils.

    The Journal of antibiotics·1988
    Same author

    Biological evaluation of stability of 5-azacytidine.

    The Journal of antibiotics·1984
    Same author

    Two new polypeptide antibiotics, CC-1014 and CC-1014B.

    The Journal of antibiotics·1982
    Same author

    CC-1065 (NSC 298223), a potent new antitumor agent improved production and isolation, characterization and antitumor activity.

    The Journal of antibiotics·1981

    Area of Science:

    • Microbiology
    • Biotechnology
    • Fermentation Science

    Background:

    • Chartreusin is an antibiotic produced by Streptomyces chartreusis.
    • Understanding factors that influence chartreusin yield is crucial for its production.
    • The molecular structure of chartreusin includes d-fucose.

    Purpose of the Study:

    • To investigate methods for increasing chartreusin production.
    • To evaluate the impact of d-fucose supplementation on chartreusin titers.
    • To establish a sensitive detection method for chartreusin.

    Main Methods:

    • Fermentation of Streptomyces chartreusis.
    • Supplementation of fermentation media with d-fucose.
    • Microbiological assay using Sarcina lutea for quantification.

    Main Results:

    • Chartreusin was produced at peak concentrations of 200-300 µg/ml.
    • Incorporating d-fucose increased chartreusin titers by 200-300% or more.
    • A microbiological assay could detect 0.5-1.0 µg/ml of chartreusin.

    Conclusions:

    • D-fucose supplementation is an effective strategy to enhance chartreusin production.
    • Optimized fermentation conditions can significantly improve antibiotic yields.
    • Sensitive microbiological assays are vital for monitoring antibiotic production.

    Related Experiment Videos