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Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

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Membrane Tension Modifies Redox Loading and Release in Single Liposome Electroanalysis.

Samuel T Barlow¹, Benjamin Figueroa¹, Dan Fu¹

¹Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, United States.

Analytical Chemistry

|February 17, 2021

Summary

Liposome content release during electrochemical detection is controllable via buffer osmolarity. This study reveals liposomes may release only partial contents, with multiple releases possible per collision.

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Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties

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Published on: November 3, 2008

Area of Science:

Biophysical Chemistry
Nanotechnology
Electrochemistry

Background:

Liposomes are crucial nanocarriers for drug delivery and biosensing.
Electrochemical detection offers high sensitivity for analyzing liposome interactions.
Understanding liposome content release mechanisms is key for optimizing these applications.

Purpose of the Study:

To investigate the loading and release dynamics of 200 nm liposomes during electrochemical detection.
To explore the influence of buffer osmolarity on liposome electroporation and content release.
To elucidate the relationship between intravesicular concentration and observed electrochemical signals.

Main Methods:

Amperometry for detecting liposome collision and ferrocyanide release at a carbon-fiber microelectrode (CFE).
Stimulated Raman Scattering (SRS) for measuring intravesicular redox concentration.
Varying buffer osmolarity to study its effect on liposome behavior.

Main Results:

Buffer osmolarity non-monotonically controlled ferrocyanide release from liposomes.
SRS measurements indicated higher intravesicular redox concentration than amperometry, suggesting partial release.
Observed electrochemical signal frequencies exceeded Poisson statistics, indicating multiple release events per liposome collision.

Conclusions:

Electrochemical detection of 200 nm liposomes is feasible with high temporal resolution and sensitivity.
Liposome content release is tunable via buffer osmolarity, with partial release being a key factor.
Multiple electroporation events from single liposome collisions can occur, impacting signal interpretation.