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Circumferential trabecular meshwork cell density in the human eye.

Markus H Kuehn1, Janice A Vranka2, David Wadkins1

  • 1Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, 52242, USA; Center for the Prevention and Treatment of Visual Loss, Iowa City VA Health Care System, Iowa City, IA, 52246, USA.

Experimental Eye Research
|February 17, 2021
PubMed
Summary
This summary is machine-generated.

Trabecular meshwork (TM) cell density varies around the human eye but not with outflow. Primary open angle glaucoma (POAG) eyes show reduced TM cellularity without increased variability compared to normal eyes.

Keywords:
Aqueous humorGlaucomaHumanMorphometrySegmental outflowTrabecular meshwork

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Glaucoma Research

Background:

  • Trabecular meshwork (TM) cells are crucial for maintaining ocular tissue and regulating intraocular pressure (IOP).
  • Understanding TM cell distribution and density is vital for glaucoma research, particularly primary open angle glaucoma (POAG).

Purpose of the Study:

  • To investigate TM cell distribution along the human eye's circumference.
  • To determine differences in TM cell density between high and low outflow regions.
  • To compare TM cell distribution in POAG eyes versus normal eyes.

Main Methods:

  • Histochemical analysis of TM cell density in 12 radial segments from human donor eyes (n=6) with and without POAG.
  • Mapping high, median, and low outflow areas in perfused human donor eyes (n=11 normal, n=6 POAG).
  • Determining TM cell densities in these mapped outflow regions.

Main Results:

  • Average TM cell density negatively correlated with maximum IOP (R²=0.91).
  • Significant TM cell density variations exist along the eye's circumference and within segments, but not between normal and POAG eyes.
  • TM cell density did not correlate with segmental outflow, and POAG eyes had reduced TM cellularity but similar variability to normal eyes.
  • Measurements from 12 sections across 2 segments reliably estimate human eye TM cell density.

Conclusions:

  • TM cell density exhibits significant circumferential variation within an eye, independent of outflow.
  • While POAG eyes show reduced TM cellularity, their TM cell distribution variability is comparable to normal eyes.
  • A cost-effective method using 12 sections from 2 segments can reliably estimate human eye TM cell density.