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Related Experiment Video

Updated: Nov 17, 2025

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

Base editing and prime editing in laboratory animals.

Federico Caso¹, Benjamin Davies¹

¹Wellcome Centre for Human Genetics, 6396University of Oxford, UK.

Laboratory Animals

|February 18, 2021

Summary

Genome editing technologies like CRISPR have advanced laboratory animal research. Newer methods, base editing and prime editing, offer improved precision and broader applications for genetic engineering in animals.

Keywords:

Animal models CRISPR adenine base editing base editing cytosine base editing gene therapy prime editing transgenesis

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Area of Science:

Genetics
Molecular Biology
Bioengineering

Background:

Programmable RNA-dependent Cas endonucleases revolutionized genome engineering.
Conventional CRISPR editing induces DNA double-strand breaks (DSBs), raising precision concerns.
Iterations like base editing were developed to improve editing precision without DSBs.

Purpose of the Study:

To review advancements in genome editing technologies for laboratory animal research.
To highlight the precision and applications of base editing and prime editing.
To discuss the potential of new genome editing tools in animal models and gene therapy.

Main Methods:

Review of base editing (cytosine and adenine) applications in animal models.
Analysis of prime editing as a 'search and replace' genome manipulation tool.
Examination of genome editing precision and limitations in laboratory animal research.

Main Results:

Base editing enables precise single base conversions without DSBs, successfully applied in zygotes and somatic cells.
Cytosine and adenine base editors facilitate animal model generation and gene therapy development.
Prime editing offers potential for wider genomic manipulations beyond base editing's scope.

Conclusions:

Base editing provides precise genome editing for animal models and gene therapy.
Prime editing represents a significant advancement, potentially overcoming limitations of previous methods.
These genome editing tools are crucial for future laboratory animal research and therapeutic development.