Molecular detection of Babesia microti in dogs and cat blood samples collected from Punjab (Pakistan)

Babesia microti (B. microti) is an intra erythrocytic protozoan that mainly infects red blood cells and causes babesiosis. Its frequent hosts are rodents, ticks and humans. Dog's blood samples (N = 150) were collected from three different districts in Punjab including Multan (N = 49), Islamabad/Rawalpindi (N = 49) and Lahore (N = 52) while 159 cat's blood samples were collected from Lahore (N = 159). Data on the epidemiological characters of all animals (including age, gender, breed, body temperature, deworming, vaccination, mucus membrane status, hydration status, presence of hematuria and tick infestation) was collected through questionnaire. Polymerase chain reaction (PCR) amplified a 238 base pair amplicon specific for 18S rRNA gene of B. microti in two (1.3%) dog and 21 (13.2%) cat blood samples. Amplified PCR products were confirmed by DNA sequencing and the four partial 18S rRNA gene sequences were submitted to the EMBL/GenBank. Among epidemiological factors, high body temperature (P < 0.05) and pale mucous membrane (P < 0.05) were the parameters associated with the presence of B. microti in infected dogs. Females were found to be more infected (P = 0.05) than males and incidence of B. microti infection was higher in cat samples collected during winter months (P = 0.0001) than in summer. In conclusion, we are reporting the prevalence of B. microti in blood samples of cats and dogs from Pakistan for the first time and recommending that this Protozoan parasite should be considered for screening in cats and dogs with compatible clinical signs.