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Lactobacillus plantarum 299v probiotic supplementation in men with stable coronary artery disease suppresses systemic

Benjamin C Hofeld1, Venkata K Puppala1, Sudhi Tyagi1

  • 1Department of Medicine, Division of Cardiovascular Medicine, Medical College of Wisconsin, Milwaukee, WI, USA.

Scientific Reports
|February 18, 2021
PubMed

Insights

Lactobacillus plantarum 299v (Lp299v) supplementation reduced inflammation and improved vascular function in coronary artery disease (CAD) patients. Lp299v induced anti-inflammatory gene expression changes in human cells, benefiting CAD patients.

Area of Science:

  • Cardiovascular Medicine
  • Immunology
  • Microbiology

Background:

  • Systemic anti-inflammatory therapy benefits coronary artery disease (CAD) patients.
  • Lactobacillus plantarum 299v (Lp299v) supplementation improved vascular endothelial function in men with stable CAD.
  • The anti-inflammatory mechanisms of Lp299v in CAD remain unclear.

Purpose of the Study:

  • To investigate if Lp299v's beneficial effects on vascular function in CAD patients are mediated by anti-inflammatory actions.
  • To analyze the impact of Lp299v supplementation on the transcriptome of peripheral blood mononuclear cells (PBMCs).
  • To correlate Lp299v-induced transcriptome changes with improvements in vascular function.

Main Methods:

  • Plasma from CAD patients before and after Lp299v supplementation was incubated with healthy donor PBMCs.
  • PBMC transcriptomes were analyzed, and gene ontology analyses were performed.
  • Lp299v-induced transcriptome changes were compared with changes in brachial flow-mediated dilation (FMD).
  • Daily alcohol users (DAUs) were analyzed separately.

Main Results:

  • In non-DAU subjects, Lp299v improved FMD and reduced circulating IL-8, IL-12, and leptin.
  • 997 genes in PBMCs showed significant changes post-Lp299v exposure, indicating strong anti-inflammatory effects.
  • Pathway analysis revealed downregulation of IL-1β, interferon-stimulated, and toll-like receptor signaling pathways, with increased regulatory T-cell (Treg) activity.
  • Gene expression changes, including reduced GBP1, JAK2, and TRAIL, correlated with improved FMD.

Conclusions:

  • Lp299v supplementation induces anti-inflammatory transcriptome changes in human PBMCs from non-DAU men with stable CAD.
  • These transcriptomic alterations may contribute to the observed improvements in vascular function.
  • Further research is needed to identify specific circulating metabolites responsible for these effects.

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