Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcription clusters and developmental pathways - nature, nurture, noise.

Journal of cell science·2026
Same author

Full Vectorial Field Sensing Using Liquid Crystal Droplet Arrays.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

On the molecular basis of enduring memory in neurons, and cell fate in fibroblasts.

Nucleic acids research·2026
Same author

Generation of Clonal Cultures of Adherent or Suspension Cells Using Flat Sessile Drops for Assurance of Monoclonality.

Biotechnology and bioengineering·2025
Same author

DNA replication timing reveals genome-wide features of transcription and fragility.

Nature communications·2025
Same author

Bridging-mediated compaction of mitotic chromosomes.

Nucleus (Austin, Tex.)·2025
Same journal

Parallelized contactless microfluidic dispenser with superhydrophobic nozzles for scalable combinatorial screening.

Biomicrofluidics·2026
Same journal

Time resolved luminescence of millisecond lifetime dyes in droplet microfluidic systems.

Biomicrofluidics·2026
Same journal

Emerging trends in functional molecularly imprinted polymers for electrochemical detection of biomarkers.

Biomicrofluidics·2025
Same journal

Deep learning assisted mechanotyping of individual cells through repeated deformations and relaxations in undulating channels.

Biomicrofluidics·2025
Same journal

<i>Giardia</i> purification from fecal samples using rigid spiral inertial microfluidics.

Biomicrofluidics·2025
Same journal

Point of care sepsis diagnosis: Exploring microfluidic techniques for sample preparation, biomarker isolation, and detection.

Biomicrofluidics·2025
See all related articles

Related Experiment Video

Updated: Nov 17, 2025

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
07:47

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Published on: February 10, 2023

2.0K

Creating wounds in cell monolayers using micro-jets.

Cristian Soitu1, Mirela Panea2, Alfonso A Castrejón-Pita3

  • 1Osney Thermofluids Institute, Department of Engineering Science, University of Oxford, Osney Mead, Oxford OX2 0ES, United Kingdom.

Biomicrofluidics
|February 18, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a novel, contactless method for creating precise wounds in cell cultures using micro-jets. This technique simplifies wound healing assays, making them more accessible and efficient for biological research.

More Related Videos

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
05:46

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate

Published on: March 7, 2017

10.9K
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
08:55

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Published on: March 17, 2018

22.6K

Related Experiment Videos

Last Updated: Nov 17, 2025

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
07:47

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Published on: February 10, 2023

2.0K
A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
05:46

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate

Published on: March 7, 2017

10.9K
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
08:55

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Published on: March 17, 2018

22.6K

Area of Science:

  • Cell Biology
  • Biomedicine
  • Biotechnology

Background:

  • Traditional wound healing assays are often labor-intensive and require expensive equipment.
  • There is a need for more accessible and efficient methods in cell biology research.

Purpose of the Study:

  • To develop a novel, contactless method for creating wounds in cell monolayers.
  • To enable automation and multiplexing of wound healing assays using standard laboratory equipment.

Main Methods:

  • Utilized micro-jets of cell media or fluorocarbon (FC40) to create 2D wound patterns.
  • Developed a method using immiscible liquids (media and FC40) in Petri dishes to form microchambers.
  • Integrated microjet wounding with microchamber arrays for automated and multiplexed cell culture experiments.

Main Results:

  • Successfully created wounds with arbitrary 2D patterns contactlessly.
  • Demonstrated a method for forming isolated microchambers within standard Petri dishes.
  • Established a workflow compatible with existing biological laboratory incubators and microscopes.

Conclusions:

  • The described contactless microjet method offers a versatile and cost-effective approach to wound creation in cell monolayers.
  • The integration with microchamber arrays allows for automated and multiplexed wound healing studies.
  • This assay is expected to have broad applications in wound healing research and drug screening.