Related Experiment Video
Updated: Nov 17, 2025
10:21
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
Published on: May 5, 2016
11.0K
Amplified Fluorescence by Hollow-Porous Plasmonic Assembly: A New Observation and Its Application in Multiwavelength
Kai-Xin Xie1, Qian Liu2, Xiu-Li Song1
1Department of Chemistry, Taiyuan Normal University, Jinzhong, Shanxi 030619, PR China.
Analytical Chemistry
|February 18, 2021
Summary
Hollow-porous gold nanocages significantly boost surface plasmon coupled emission (SPCE) sensitivity. This novel approach enhances fluorescence signals by over 600 times, overcoming limitations of traditional SPCE for improved analytical detection.
Area of Science:
- Nanotechnology
- Analytical Chemistry
- Optics
Background:
- Surface plasmon coupled emission (SPCE) offers directional radiation but lacks sensitivity.
- Hollow-porous plasmonic nanoparticles present a potential solution for enhancing SPCE.
- Existing SPCE methods face signal quenching due to resonance energy transfer.
Purpose of the Study:
- To investigate the use of hollow-porous gold nanocages (AuNCs) for enhancing SPCE.
- To overcome the sensitivity limitations of conventional SPCE techniques.
- To develop an amplified SPCE system for simultaneous multi-analyte detection.
Main Methods:
- Assembly of hollow-porous gold nanocages (AuNCs).
- Integration of AuNCs with fluorophores for SPCE measurements.
- Utilizing mixed hollow nanoparticles (AuNCs and gold nanoshells) for multiwavelength detection.
Main Results:
- AuNCs enhanced SPCE signals by over 150 times and free-space emission by over 600 times.
- Enhanced SPCE overcomes signal quenching observed in normal SPCE.
- The enhancement is attributed to wavelength matching, electromagnetic field enhancement, and new radiation channels.
Conclusions:
- Hollow-porous AuNCs effectively enhance SPCE, significantly improving detection sensitivity.
- The developed amplified SPCE system enables simultaneous detection and imaging of multiple analytes.
- This approach broadens the applicability of SPCE in sensing and imaging.
Related Concept Videos
01:37Super-resolution Fluorescence Microscopy
11.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.9K
01:14Photoluminescence: Applications
699
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
699