Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.9K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.9K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.9K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Daily Diary Study of Positive Experiences and Alcohol Use Among Non-Abstaining Adults Aged 50+ Years.

Journal of aging and health·2026
Same author

High-intensity and blackout drinking impact on next-day cognitive functioning among college-attending young adults.

Alcohol, clinical & experimental research·2026
Same author

Diagnostic challenge in veterinary pathology: A large vesicle-containing, membrane-bound structure in the myocardium of a cat with cardiomyopathy.

Veterinary pathology·2026
Same author

Longitudinal changes in alcohol use across 20 years of adulthood: a U.S. National daily diary study.

Aging & mental health·2025
Same author

Acute Next-Day Effects of Alcohol Use on Daily Cognitive Functioning Among Young Adults: Protocol for a 21-Day Diary Study.

JMIR research protocols·2025
Same author

Rickettsia lanei, sp. nov. (Rickettsiales: Rickettsiaceae), a Newly Recognized Pathogen of Humans Associated with the Rabbit Tick, Haemaphysalis leporispalustris (Acari: Ixodidae).

The American journal of tropical medicine and hygiene·2025

Related Experiment Video

Updated: Nov 17, 2025

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
09:18

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Published on: September 7, 2018

81.5K

Difficulties in Differentiating Coronaviruses from Subcellular Structures in Human Tissues by Electron Microscopy.

Hannah A Bullock, Cynthia S Goldsmith, Sherif R Zaki

    Emerging Infectious Diseases
    |February 18, 2021
    PubMed
    Summary

    Transmission electron microscopy is crucial for identifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This study details distinguishing features of SARS-CoV-2 particles from cellular structures to improve COVID-19 diagnostics.

    Keywords:
    COVID-19SARSSARS-CoV-2coronaviruscoronavirus diseaseelectron microscopyrespiratory infectionssevere acute respiratory syndrome coronavirus 2ultrastructureviruseszoonoses

    More Related Videos

    Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
    08:31

    Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

    Published on: July 20, 2022

    3.5K
    Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
    08:29

    Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

    Published on: October 21, 2014

    12.4K

    Related Experiment Videos

    Last Updated: Nov 17, 2025

    Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
    09:18

    Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

    Published on: September 7, 2018

    81.5K
    Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
    08:31

    Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

    Published on: July 20, 2022

    3.5K
    Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
    08:29

    Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

    Published on: October 21, 2014

    12.4K

    Area of Science:

    • Pathology
    • Microscopy
    • Virology

    Background:

    • The COVID-19 pandemic has increased reliance on transmission electron microscopy (TEM) for SARS-CoV-2 detection.
    • Misidentification of cellular structures as SARS-CoV-2 has occurred in pathology assessments.

    Purpose of the Study:

    • To provide clear morphological criteria for distinguishing SARS-CoV-2 particles from subcellular structures in TEM images.
    • To enhance the accuracy of SARS-CoV-2 identification in patient tissues.

    Main Methods:

    • Detailed morphological analysis of coronavirus particles using transmission electron microscopy.
    • Comparison of viral particle characteristics with known subcellular structures.

    Main Results:

    • SARS-CoV-2 particles range from 60-140 nm and are found within membrane-bound vacuoles.
    • Nucleocapsids appear as 6-12 nm dense dots in cross-section.
    • Surface spikes are more visible in extracellular particles or negative stain preparations than in thin sections.

    Conclusions:

    • Accurate identification of SARS-CoV-2 requires differentiating viral morphology from cellular components.
    • Established morphological features aid in reliable detection of SARS-CoV-2 in tissues via TEM.
    • Improved diagnostic accuracy supports better understanding of COVID-19 pathology.