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A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
Published on: July 5, 2017
High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells
Ruiqi Ma1, Lu Gan1, Sanjie Jiang2
1Department of Ophthalmology, Fudan Eye & ENT Hospital, Shanghai, China; Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China.
Abstract:
The angiotensin-converting enzyme 2 (ACE2) receptor has been proved for SARS-CoV-2 cell entry after auxiliary cellular protease priming by transmembrane protease serine 2 (TMPRSS2), but the co-effect of this molecular mechanism was unknown. Here, single-cell sequencing was performed with human conjunctiva and the results have shown that ACE2 and TMPRSS2 were highly co-expressed in the goblet cells with genes involved in immunity process. This identification of conjunctival cell types which are permissive to virus entry would help to understand the process by which SARS-CoV-2 infection was established. These finding might be suggestive for COVID-19 control and protection.
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