Related Experiment Videos
Phosphorylation of the 90 kDa heat shock protein in heat shocked HeLa cell lysates
V Legagneux1, M F Dubois, M Morange
1Groupe de Biologie Moléculaire du Stress, Institut Pasteur, Paris, France.
FEBS Letters
|April 25, 1988
Abstract:
The 90 kDa heat shock protein (hsp 90) is a major phosphorylated protein under normal growth conditions. However, it does not incorporate detectable levels of phosphate by incubation of control HeLa cell lysates with [gamma-32P]ATP in vitro. In this paper we show that strong phosphorylation of hsp 90 occurs in lysates prepared from heat shocked HeLa cells. Possible involvement of the eukaryotic initiation factor 2 kinase of the heme-controlled repressor of translation is discussed.