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Related Experiment Videos

Serological responses in experimental bovine tuberculosis.

A W Lepper, L A Corner, C W Pearson

    Australian Veterinary Journal
    |July 1, 1977
    PubMed
    Summary

    This study tracked bovine tuberculosis (M. bovis) in heifers, finding varied disease severity and unreliable antibody test results. Early M. bovis infection can cause severe illness and detectable bacteria in the blood.

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    Veterinary immunology and immunopathology·1997

    Area of Science:

    • Veterinary Medicine
    • Infectious Diseases
    • Immunology

    Background:

    • Bovine tuberculosis, caused by Mycobacterium bovis (M. bovis), poses a significant threat to cattle health and global food security.
    • Accurate diagnostic tools and understanding disease progression are crucial for controlling M. bovis infections in cattle.

    Purpose of the Study:

    • To investigate the clinical, haematological, and microbiological manifestations of M. bovis infection in cattle.
    • To evaluate the efficacy of indirect immunofluorescent antibody (IFA) and bentonite flocculation tests in detecting M. bovis infection.

    Main Methods:

    • Five two-year-old heifers were intravenously inoculated with M. bovis strain AN5.
    • Clinical signs, haematological parameters, and microbiological samples were monitored throughout the experiment.
    • Antibody levels were measured using IFA and bentonite flocculation tests before and after infection.

    Main Results:

    • All heifers developed M. bovis infection, ranging from peracute pneumonia to chronic generalised tuberculosis.
    • Severe haematological changes, including leucopenia and lymphopenia, were observed in peracute and acute cases.
    • Bacteraemia was detected in peracute cases 22 days post-infection.
    • Anti-mycobacterial antibodies were detected in all infected cattle, but test sensitivity varied; only 18/61 samples were positive by both IFA and bentonite flocculation tests.
    • IgM was the predominant antibody detected by IFA, showing cross-reactivity with M. avium.

    Conclusions:

    • M. bovis infection in cattle presents with a wide spectrum of clinical severity.
    • Current antibody-based diagnostic tests (IFA and bentonite flocculation) showed limited sensitivity and specificity for M. bovis detection in this study.
    • The cross-reactivity of detected IgM antibodies with M. avium suggests potential challenges in differentiating M. bovis from other mycobacterial infections using these methods.

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