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Syzygium Polyanthum Reduced TNF-α and ADAM17 Protein Expression in Myocardial Infarction Rat Model
Refli Hasan1, Gontar Alamsyah Siregar1, Dharma Lindarto1
1Department of Internal Medicine, School of Medicine, Universitas Sumatera Utara, Medan, Indonesia.
Background:
In myocardial infarction (MI), inflammatory cytokine including tumor necrosis factor-α (TNF-α) plays pivotal role throughout worsening processes and recovery, whereas its cleavage is controlled by a disintegrin and metalloproteinases-17 (ADAM-17). Syzygium polyanthum (Wight) is widely used plant in Indonesia and Southeast Asian countries to treat various illnesses.
Objective:
This study aimed to analyze the effect of S. polyanthum extract towards TNF-α and ADAM17 expressions in MI rat model.
Methods:
Myocardial infarction were surgically induced in wistar rats by ligating left anterior descending coronary artery in both treatment and control group. Around 3.6 mg of S. polyanthum extract (SPE) was administered via nasogastric tube to treatment groups, while control group received only saline. Serum TNF-α level and expression of TNF-α and ADAM17 in blanching heart muscle was examined in both groups on day 1, day 4, day 7, and day 14 after treatment with SPE.
Results:
Reduction of serum TNF-α levels was markedly seen from day 4 in treatment group and was lower than in control group since day 4. Expression of ADAM17 was reduced and expression of TNF-α was only seen in myocardial membrane in group with SPE treatment.
Conclusion:
Syzygium polyanthum exerts its anti-inflammatory effect by decreasing ADAM17 expression subsequently lowering TNF-α regulation in MI rat model.
Insights
Syzygium polyanthum extract reduced inflammatory tumor necrosis factor-alpha (TNF-α) and ADAM17 expression in a myocardial infarction (MI) rat model, showcasing its anti-inflammatory potential.
Area of Science:
- Cardiovascular Research
- Pharmacology
- Traditional Medicine
Background:
- Myocardial infarction (MI) involves inflammatory cytokines like tumor necrosis factor-alpha (TNF-α), whose activity is regulated by ADAM17.
- Syzygium polyanthum (SPE) is a traditional remedy in Southeast Asia with potential therapeutic applications.
Purpose of the Study:
- To investigate the impact of Syzygium polyanthum extract on TNF-α and ADAM17 expression in a rat model of MI.
- To elucidate the anti-inflammatory mechanisms of SPE in the context of cardiac injury.
Main Methods:
- Myocardial infarction was induced in Wistar rats via coronary artery ligation.
- Rats received either 3.6 mg of SPE or saline (control) via nasogastric tube.
- Serum TNF-α levels and myocardial expression of TNF-α and ADAM17 were assessed on days 1, 4, 7, and 14 post-treatment.
Main Results:
- SPE administration significantly reduced serum TNF-α levels starting from day 4 compared to the control group.
- A notable decrease in ADAM17 expression was observed in the SPE-treated group.
- TNF-α expression was localized to the myocardial membrane in rats treated with SPE.
Conclusions:
- Syzygium polyanthum extract demonstrates anti-inflammatory effects in an MI rat model.
- SPE appears to exert its benefits by downregulating ADAM17 expression, leading to reduced TNF-α levels.
- This study supports the traditional use of Syzygium polyanthum for managing inflammatory conditions like MI.
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