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Eif2s3y Promotes the Proliferation of Spermatogonial Stem Cells by Activating ERK Signaling

Mengfei Zhang1, Na Li1, Wenqing Liu1

  • 1College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.

Stem Cells International
|February 19, 2021
PubMed

Insights

Restoring male fertility after cancer treatment is crucial. This study found that overexpressing the Eif2s3y gene promotes spermatogonial stem cell proliferation and survival, offering a new path for fertility restoration in dairy goats and potentially other mammals.

Area of Science:

  • Reproductive Biology
  • Molecular Genetics
  • Cell Biology

Background:

  • Cancer treatments like chemotherapy and radiotherapy can impair male fertility.
  • Spermatogonial stem cell transplantation is a promising fertility restoration method, but cell survival remains a challenge.
  • The role of Eukaryotic translation initiation factor 2 subunit 3 and structural gene Y-linked (Eif2s3y) in spermatogenesis, especially in large animals, is not well understood.

Purpose of the Study:

  • To investigate the function of the Eif2s3y gene in dairy goat spermatogenesis.
  • To explore the potential of Eif2s3y in enhancing spermatogonial stem cell proliferation and transplantation efficiency.
  • To elucidate the molecular mechanism by which Eif2s3y influences male fertility.

Main Methods:

  • Obtained the CDS sequence of the Eif2s3y gene from dairy goat testes.
  • Analyzed Eif2s3y expression in testes and its evolutionary conservation.
  • Overexpressed Eif2s3y in dairy goat spermatogonial stem cells and assessed proliferation.
  • Investigated the involvement of the ERK signaling pathway.
  • Evaluated the effect of overexpressing Eif2s3y on transplanted goat spermatogonial stem cells in infertile mice.

Main Results:

  • The Eif2s3y gene is highly expressed in dairy goat testes and is evolutionarily conserved.
  • Overexpression of Eif2s3y significantly promoted the proliferation of dairy goat spermatogonial stem cells.
  • Eif2s3y overexpression activated the ERK signaling pathway in spermatogonial stem cells.
  • Transplanted goat spermatogonial stem cells overexpressing Eif2s3y showed enhanced survival and colony formation in infertile mice.

Conclusions:

  • Eif2s3y plays a crucial role in promoting dairy goat spermatogonial stem cell proliferation and survival.
  • Activating the ERK signaling pathway is a key mechanism for Eif2s3y's function in spermatogenesis.
  • Eif2s3y holds significant potential for improving spermatogonial stem cell transplantation efficiency for male fertility restoration.
  • This research provides a foundation for utilizing Eif2s3y in reproductive technologies, including the development of surrogate sires for germplasm regeneration.

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