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Pigmentation changes in brevibacteria induced by mutagenic treatment
M Konícková-Radochová1, J Konícek, V Rytír
1Department of Microbial Genetics, Czechoslovak Academy of Sciences, Prague.
Folia Microbiologica
|January 1, 1988
Summary
Mutagenesis of Brevibacterium strains induced new pigmentation types and altered amino acid production. This research explores genetic modification for novel bacterial traits and L-lysine biosynthesis.
Area of Science:
- Microbiology
- Bacterial Genetics
Background:
- Pigmentation in Brevibacterium species can be altered through mutagenesis.
- Understanding genetic variability in bacterial strains is crucial for biotechnological applications.
Purpose of the Study:
- To investigate inducible pigmentation changes in Brevibacterium sp. M27 and B. flavum using N-methyl-N'-nitro-N-nitrosoguanidine.
- To characterize the resulting pigmented mutants for antibiotic resistance and amino acid production.
Main Methods:
- Treatment of bacterial strains with N-methyl-N'-nitro-N-nitrosoguanidine.
- Selection of mutants on antibiotic-containing media.
- Analysis of pigmentation, antibiotic resistance, and amino acid production.
Main Results:
- Inducible pigmentation changes resulted in white, pink, and orange variants from yellow parent strains.
- Mutants exhibited resistance to tetracycline or streptomycin.
- Multiple mutant strains showed altered L-lysine production, with some exhibiting variable or no production, and others showing limited production of other amino acids.
Conclusions:
- N-methyl-N'-nitro-N-nitrosoguanidine is effective in inducing pigmentation changes and genetic alterations in Brevibacterium.
- Pigmented mutants can be selected for antibiotic resistance.
- Genetic modifications impact L-lysine biosynthesis and other amino acid production pathways in Brevibacterium.