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Updated: Nov 17, 2025

The Soft Agar Colony Formation Assay
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ZBTB20 regulates WNT/CTNNB1 signalling pathway by suppressing PPARG during hepatocellular carcinoma tumourigenesis

Jeffrey C To1, Amy P Chiu1, Barbara R Tschida2

  • 1Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR.

JHEP Reports : Innovation in Hepatology
|February 19, 2021
PubMed
Abstract

Keywords:
AFP, alpha-foetoproteinBTB/POZ, broad complexCTNNB1CTNNB1, catenin beta 1Fah, fumarylacetoacetate hydrolaseGSK3B, glycogen synthase kinase 3 betaHCC, hepatocellular carcinomaHHL, immortalized human hepatic cell lineHepatocellular carcinomaIF, immunofluorescenceNTBC, 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedioneOFP, orange fluorescent proteinPHI, post-hydrodynamic injectionPOK, POZ and KruppelPPARGPPARG, peroxisome proliferator activated receptor gammaReverse genetic screenSB, Sleeping BeautySleeping BeautyZBTB20ZBTB20, zinc finger and BTB domain containing 20qPCR, quantitative RT-PCRtramtrack, bric a brac/poxvirus and zinc finger

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