Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

10.4K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
10.4K
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

263
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
263
2° Amines to N-Nitrosamines: Reaction with NaNO201:20

2° Amines to N-Nitrosamines: Reaction with NaNO2

4.9K
Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
4.9K
Preparation of Amines: Reduction of Oximes and Nitro Compounds01:29

Preparation of Amines: Reduction of Oximes and Nitro Compounds

4.3K
Oximes can be reduced to primary amines using catalytic hydrogenation, hydride reduction, or sodium metal reduction. The reduction of aliphatic and aromatic nitro compounds to primary amines takes place by either catalytic hydrogenation or by using active metals like Fe, Zn, and Sn in the presence of an acid.
Though catalytic hydrogenation can reduce nitrobenzenes, the reduction is nonselective in the presence of other functional groups. For instance, if nitrobenzene contains an aldehyde group,...
4.3K
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview01:26

1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview

3.6K
Nitrous acid and nitric acids are two types of acids containing nitrogen, among which nitrous acid is weaker than nitric acid. Nitrous acid with a pKa value of 3.37 ionizes in water to give a nitrite ion and the hydronium ion.
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...
3.6K
The Nitrogen Cycle01:49

The Nitrogen Cycle

58.4K
Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
58.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PTP1B deficiency in the reward system suppressed the rewarding value of a high-fat diet.

Brain research bulletin·2026
Same author

HDA19-mediated deacetylation of histone H3.3 at lysines 27 and 36 regulates plant sensitivity to salt stress.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Nutrient sensing and transceptor-mediated metabolic control in yeast.

FEMS yeast research·2026
Same author

FLOWERING PROMOTING FACTOR1 Family Proteins Coordinate Seasonal Growth and Development.

Plant & cell physiology·2026
Same author

GA20ox1-mediated GA4 production promotes inflorescence stem growth in Arabidopsis via inner-layer cell proliferation.

Plant & cell physiology·2026
Same author

Multiple ammonium transporters in fission yeast are coordinated by transcriptional and localization regulation in response to nitrogen starvation.

Journal of cell science·2026

Related Experiment Video

Updated: Nov 17, 2025

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

18.3K

The analytical method to identify the nitrogen source for nitric oxide synthesis.

Ryo Nasuno1, Yuki Yoshikawa1, Hiroshi Takagi1

  • 1Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Takayama-cho, Ikoma, Nara, Japan.

Bioscience, Biotechnology, and Biochemistry
|February 19, 2021
PubMed
Summary

Researchers developed a method using stable isotope labeling and mass spectrometry to identify the nitrogen source for nitric oxide (NO) generation. This technique successfully traced NO production from labeled nitrite in both lab experiments and living organisms.

Keywords:
fluorescence probemass spectrometrynitric oxidenitrogen sourcestable isotope

More Related Videos

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
07:14

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

11.9K
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.5K

Related Experiment Videos

Last Updated: Nov 17, 2025

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

18.3K
Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
07:14

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

11.9K
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.5K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Physiology

Background:

  • Nitric oxide (NO) is a crucial signaling molecule involved in numerous physiological processes.
  • Understanding the nitrogen sources for NO synthesis is vital for comprehending its biological roles.
  • Existing methods for tracing NO origins can be limited in scope and sensitivity.

Purpose of the Study:

  • To develop and validate a novel analytical method for identifying the specific nitrogen source utilized in nitric oxide (NO) generation.
  • To apply this method for distinguishing NO production pathways in both in vitro and in vivo settings.

Main Methods:

  • Development of an analytical technique combining liquid chromatography with tandem mass spectrometry (LC-MS/MS).
  • Incorporation of stable isotope labeling (using 15N) to trace nitrogen incorporation into NO.
  • Application of the method to detect 15N-labeled NO-containing compounds derived from 15N-labeled nitrite.

Main Results:

  • The developed LC-MS/MS method with stable isotope labeling successfully identified 15N-labeled NO-containing compounds.
  • The method confirmed that 15N-labeled nitrite serves as a direct substrate for NO generation in vitro.
  • The technique also demonstrated in vivo traceability of NO production from labeled nitrite.

Conclusions:

  • A robust analytical method for tracing nitrogen sources in NO synthesis has been established.
  • This technique provides a valuable tool for investigating NO metabolism and signaling pathways.
  • The findings highlight the utility of stable isotope labeling in conjunction with mass spectrometry for biological tracer studies.