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Related Experiment Video

Updated: Nov 16, 2025

Author Spotlight: Time and Cost-Effective Fibrinogen-PAGE for Fibrinogenolytic Studies

Author Spotlight: Time and Cost-Effective Fibrinogen-PAGE for Fibrinogenolytic Studies

Published on: April 19, 2024

Fibrinogen heterogeneity in horses.

Elise B Russell¹, Natalie F Courtman¹, Leilani L Santos¹

¹U-Vet Werribee Animal Hospital and Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria, Australia.

Journal of Veterinary Internal Medicine

|February 19, 2021

Summary

Fibrinogen heterogeneity, a variation in fibrinogen structure, has been confirmed in horses. This finding is significant for accurate fibrinogen measurements in equine plasma.

Keywords:

EDTA Vila method citrate equine fibrinogen variants high molecular weight fibrinogen low molecular weight fibrinogen protein purification

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Last Updated: Nov 16, 2025

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Published on: April 19, 2024

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A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood

A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood

Published on: August 15, 2025

Area of Science:

Veterinary Hematology
Biochemistry
Equine Medicine

Background:

Fibrinogen heterogeneity is documented in humans, impacting measurements via the modified Clauss assay.
The presence of fibrinogen heterogeneity in horses remained uninvestigated.

Purpose of the Study:

To investigate and confirm the existence of fibrinogen heterogeneity in horses.

Main Methods:

Purification of presumed fibrinogen from pooled equine plasma.
Analysis using gel electrophoresis, Western blotting with anti-human fibrinogen antiserum, and liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Main Results:

Gel electrophoresis revealed two distinct protein bands in nonreduced equine fibrinogen, corresponding to high (approx. 377 kDa) and low (approx. 318 kDa) molecular weight fractions.
Western blotting and LC-MS/MS analyses confirmed these bands as equine fibrinogen.

Conclusions:

Fibrinogen heterogeneity is present in horses.
This heterogeneity may influence diagnostic assays used for equine fibrinogen quantification.