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Fibrinogen heterogeneity in horses
Elise B Russell1, Natalie F Courtman1, Leilani L Santos1
1U-Vet Werribee Animal Hospital and Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria, Australia.
Journal of Veterinary Internal Medicine
|February 19, 2021
Summary
Fibrinogen heterogeneity, a variation in fibrinogen structure, has been confirmed in horses. This finding is significant for accurate fibrinogen measurements in equine plasma.
Area of Science:
- Veterinary Hematology
- Biochemistry
- Equine Medicine
Background:
- Fibrinogen heterogeneity is documented in humans, impacting measurements via the modified Clauss assay.
- The presence of fibrinogen heterogeneity in horses remained uninvestigated.
Purpose of the Study:
- To investigate and confirm the existence of fibrinogen heterogeneity in horses.
Main Methods:
- Purification of presumed fibrinogen from pooled equine plasma.
- Analysis using gel electrophoresis, Western blotting with anti-human fibrinogen antiserum, and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Main Results:
- Gel electrophoresis revealed two distinct protein bands in nonreduced equine fibrinogen, corresponding to high (approx. 377 kDa) and low (approx. 318 kDa) molecular weight fractions.
- Western blotting and LC-MS/MS analyses confirmed these bands as equine fibrinogen.
Conclusions:
- Fibrinogen heterogeneity is present in horses.
- This heterogeneity may influence diagnostic assays used for equine fibrinogen quantification.
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