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Temperature-Controlled Adhesion to Carbohydrate Functionalized Microgel Films: An E. coli and Lectin Binding Study.

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Summary

Thermoresponsive poly(N-isopropylacrylamide) microgels functionalized with mannose exhibit temperature-dependent binding. Binding of concanavalin A (ConA) and E. coli varies with temperature, influenced by receptor size and microgel swelling.

Keywords:
PNIPAMbiomimetic hydrogelsglycocalyxlower critical solution temperaturemicrogel coatingsmultivalencyresponsive material

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Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Microfluidics

Background:

  • Poly(N-isopropylacrylamide) (PNIPAM) microgels display thermoresponsive behavior, undergoing a phase transition at their lower critical solution temperature (LCST).
  • Mannose functionalization on microgel surfaces creates specific binding sites for lectins like concanavalin A (ConA).
  • Understanding the influence of temperature on molecular interactions at the microgel interface is crucial for developing smart materials.

Purpose of the Study:

  • To prepare and characterize thermoresponsive mannose-functionalized PNIPAM microgel monolayers.
  • To investigate the specific binding of concanavalin A (ConA) and E. coli to these microgels above and below the LCST.
  • To elucidate the impact of temperature-induced microgel swelling/collapse on binding kinetics and affinity.

Main Methods:

  • Preparation of mannose-functionalized PNIPAM microgel monolayers.
  • Conducting inhibition and direct binding assays at controlled temperatures.
  • Analyzing binding dynamics and affinity using techniques sensitive to surface interactions.

Main Results:

  • Concanavalin A (ConA) binding is time-dependent, initially stronger above the LCST, but increasing below the LCST with longer incubation due to enhanced diffusion and multivalent interactions in the swollen network.
  • E. coli binding is consistently enhanced above the LCST due to increased mannose density on the collapsed microgel layer.
  • E. coli, once bound above the LCST, remains bound even upon cooling below the LCST.

Conclusions:

  • Thermoresponsive mannose-functionalized microgel layers facilitate specific molecular binding.
  • The temperature-dependent transition between swollen and collapsed microgel states modulates binding affinity based on receptor size.
  • These findings highlight the potential of stimuli-responsive materials for controlled capture and release applications.