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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

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Chromo-fluorogenic probes for β-galactosidase detection.

Beatriz Lozano-Torres^1,2,3,4, Juan F Blandez^1,2,3,4, Félix Sancenón^5,6,7,8

¹Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain.

Analytical and Bioanalytical Chemistry

|February 19, 2021

Summary

Molecular probes detect beta-galactosidase (β-Gal), an enzyme linked to rare diseases and cellular senescence. These probes offer rapid, in situ detection for disease diagnosis and research advancement.

Keywords:

Cellular senescence Chromo-fluorogenic probes In vitro and in vivo detection β-Galactosidase detection

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Area of Science:

Biochemistry
Molecular Biology
Enzymology

Background:

Beta-galactosidase (β-Gal) is a crucial enzyme in human physiology.
Dysregulation of β-Gal activity is associated with rare genetic disorders and cellular senescence.
Accurate detection of β-Gal is vital for understanding and diagnosing related diseases.

Purpose of the Study:

To review advancements in molecular probes for detecting β-Gal.
To highlight probes for both in vitro and in vivo applications.
To assess the utility of these probes in disease diagnostics.

Main Methods:

Design of molecular probes based on galactopyranoside residues linked to signaling units.
Utilizing β-Gal-induced hydrolysis of glycosidic bonds to release signaling units.
Observing changes in color and/or emission upon probe activation.

Main Results:

Development of diverse molecular probes for β-Gal detection.
Probes demonstrate significant changes in optical properties upon enzymatic activity.
Successful application of probes for in situ and rapid detection.

Conclusions:

Molecular probes are effective tools for detecting β-Gal activity.
These probes facilitate the rapid diagnosis of β-Gal-related diseases.
Continued research in this area promises further diagnostic and therapeutic insights.