The Xenopus Oocyte as an Expression System for Functional Analyses of Fish Aquaporins

Aquaporins are membrane proteins present in all organisms that selectively transport water and small, uncharged solutes across biological membranes along an osmotic gradient. Recent gene editing technologies in zebrafish (Danio rerio) have started to uncover the physiological functions of the aquaporins in teleosts, but these approaches require methods to establish the effects of specific mutations on channel function. The oocytes of the South African frog Xenopus laevis are widely used for the expression of bacterial, plant, and animal aquaporins, and this heterologous system has contributed to numerous discoveries in aquaporin biology. This chapter focuses on techniques used for oocyte preparation and aquaporin expression and gives an overview of specific methods to determine water and solute permeability of the channels and their intracellular trafficking in oocytes.