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Updated: Nov 16, 2025

Human Egg Maturity Assessment and Its Clinical Application
Published on: August 19, 2019
Detection of the Polar Body After Fertilization
Hua Ruan1, Xiaogui Yi1, Honghui Huang2
1Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.
Abstract:
The polar body, with haploid DNA, is a small cell produced during the meiosis of an oocyte. Here, we describe the detailed procedures for the detection of the second polar body in zebrafish (Danio rerio) embryos after 10 min post fertilization. A polar body can be easily distinguished as a small dot with a DAPI-stained nucleus surrounded by Phalloidin-labeled F-actin in each fertilized zebrafish embryo.
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