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Related Experiment Video

Updated: Nov 16, 2025

Generating Chimeric Zebrafish Embryos by Transplantation

Generating Chimeric Zebrafish Embryos by Transplantation

Published on: July 17, 2009

Manipulating and Visualizing the Germline with Transgenic Lines.

Ding Ye^1,2, Yonghua Sun^3,4

¹State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Wuhan, China.

Methods in Molecular Biology (Clifton, N.J.)

|February 19, 2021

Summary

Researchers developed transgenic zebrafish with labeled germ cells for lifelong observation of germline development. This method allows for detailed study of primordial germ cells (PGCs) and gametes in developing gonads.

Keywords:

Confocal imaging Germline Live imaging Sample preparation Transgenic fish

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Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Published on: August 6, 2014

Area of Science:

Developmental Biology
Genetics
Zebrafish Research

Background:

Studying germline development requires methods for tracking cells throughout an organism's life.
Existing techniques may not offer continuous, high-resolution observation of germ cells from early stages to maturity.

Purpose of the Study:

To describe the generation of transgenic zebrafish (Tg(piwil1:egfp-UTRnanos3)) for germline labeling.
To detail methods for live imaging and high-resolution imaging of germ cells in zebrafish.
To provide a protocol for quantifying primordial germ cell (PGC) numbers.

Main Methods:

Generation of transgenic zebrafish line Tg(piwil1:egfp-UTRnanos3) using enhanced green fluorescent protein (eGFP) for germline-specific labeling.
Sample preparation techniques for live imaging of PGCs in early embryos.
High-resolution imaging protocols for observing germ cells within developing gonads.

Main Results:

Successful generation of transgenic zebrafish enabling continuous visualization of germline development.
Established protocols for high-resolution imaging and quantification of PGCs.
Demonstrated applicability of methods for studying germ cells in both live embryos and fixed tissues.

Conclusions:

Transgenic zebrafish with eGFP-labeled germlines offer a powerful tool for lifelong germline studies.
The described methods facilitate detailed observation and quantification of germ cells at various life stages.
These protocols are valuable for researchers studying germ cell biology and generating transgenic zebrafish for live imaging studies.